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Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors
Chapters
- 00:05Title
- 00:44Culturing mESCs
- 02:14Differentiation mESCs Toward MGE-like Telencephalic Progenitors
- 04:57Somatostatin-enriching Protocol: “DD12 GFP High Sonic Hedgehog (SHH)”
- 06:36PV-enriching Protocol: “DD11/DD17 mCherry + aPKCi”
- 07:53Generation of Nkx2.1::mCherry and Lhx6::GFP Interneuron Precursors
- 09:11Conclusion
This protocol describes a method for generating cortical interneuron progenitors and post-mitotic interneuron precursors from mouse embryonic stem cells using a modified embryoid body-to-monolayer method. These progenitors/precursors can be used in vitro or fluorescently sorted and transplanted into neonatal neocortex for studying fate determination, or used in therapeutic applications.
