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Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors
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Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors

Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors

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10:24 min

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December 03, 2017

DOI:

10.3791/56358-v

DOI:
10.3791/56358-v

•

10:24 min
December 03, 2017

•

5 Views
,
1Neuroscience Graduate Group, Perelman School of Medicine,University of Pennsylvania, 2Department of Psychiatry, Children’s Hospital of Philadelphia,University of Pennsylvania School of Medicine

Summary

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This protocol describes a method for generating cortical interneuron progenitors and post-mitotic interneuron precursors from mouse embryonic stem cells using a modified embryoid body-to-monolayer method. These progenitors/precursors can be used in vitro or fluorescently sorted and transplanted into neonatal neocortex for studying fate determination, or used in therapeutic applications.

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Mouse Embryonic Stem CellsCortical Interneuron ProgenitorsNkx2.1ParvalbuminSomatostatinMEF Feeder CellsTrypsin-EDTAGelatin-coated PlatesKSR-N2 MediaEmbryoid Bodies

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Tischfield, D. J., Anderson, S. A. Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors. J. Vis. Exp. (130), e56358, doi:10.3791/56358 (2017).

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