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Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors
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Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors
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December 03, 2017
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This protocol describes a method for generating cortical interneuron progenitors and post-mitotic interneuron precursors from mouse embryonic stem cells using a modified embryoid body-to-monolayer method. These progenitors/precursors can be used in vitro or fluorescently sorted and transplanted into neonatal neocortex for studying fate determination, or used in therapeutic applications.
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Tischfield, D. J., Anderson, S. A. Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors. J. Vis. Exp. (130), e56358, doi:10.3791/56358 (2017).
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