Dergi
/
Nörobilim
/
Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors
JoVE Journal
Nörobilim
Bu içeriği görüntülemek için JoVE aboneliği gereklidir.  Oturum açın veya ücretsiz deneme sürümünü başlatın.
JoVE Journal Nörobilim
Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors

Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors

10,150 Views

•

10:24 min

•

December 03, 2017

DOI:

10.3791/56358-v

DOI:
10.3791/56358-v

•

10:24 min
December 03, 2017

•

5 Views
,
1Neuroscience Graduate Group, Perelman School of Medicine,University of Pennsylvania, 2Department of Psychiatry, Children’s Hospital of Philadelphia,University of Pennsylvania School of Medicine

Özet

Automatically generated

This protocol describes a method for generating cortical interneuron progenitors and post-mitotic interneuron precursors from mouse embryonic stem cells using a modified embryoid body-to-monolayer method. These progenitors/precursors can be used in vitro or fluorescently sorted and transplanted into neonatal neocortex for studying fate determination, or used in therapeutic applications.

Explore More Videos

Mouse Embryonic Stem CellsCortical Interneuron ProgenitorsNkx2.1ParvalbuminSomatostatinMEF Feeder CellsTrypsin-EDTAGelatin-coated PlatesKSR-N2 MediaEmbryoid Bodies

Read Article

Cite this Article

Tischfield, D. J., Anderson, S. A. Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors. J. Vis. Exp. (130), e56358, doi:10.3791/56358 (2017).

Download .ris file

Copy

Share Video

.

Copy