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Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors

Differentiation of Mouse Embryonic Stem Cells into Cortical Interneuron Precursors

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10:24 min

December 03, 2017

DOI:

10:24 min
December 03, 2017

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Summary

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This protocol describes a method for generating cortical interneuron progenitors and post-mitotic interneuron precursors from mouse embryonic stem cells using a modified embryoid body-to-monolayer method. These progenitors/precursors can be used in vitro or fluorescently sorted and transplanted into neonatal neocortex for studying fate determination, or used in therapeutic applications.

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