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Generation of Genetically Modified Mice through the Microinjection of Oocytes
Chapters
- 00:05Title
- 00:53Synthesis of CRISPER Components
- 04:21Injection Mix and Pronuclear and Cytoplasmic Injections
- 06:31Reimplantation
- 08:21Results: Microinjection of Oocytes to Generate Genetically Modified Mice
- 09:49Conclusion
The microinjection of mouse oocytes is commonly used for both classic transgenesis (i.e., the random integration of transgenes) and CRISPR-mediated gene targeting. This protocol reviews the latest developments in microinjection, with a particular emphasis on quality control and genotyping strategies.
