/
/
Generation of Genetically Modified Mice through the Microinjection of Oocytes
Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.
Generation of Genetically Modified Mice through the Microinjection of Oocytes
20,082 Views
•
10:19 min
•
June 15, 2017
DOI:
Summary
Automatically generated
The microinjection of mouse oocytes is commonly used for both classic transgenesis (i.e., the random integration of transgenes) and CRISPR-mediated gene targeting. This protocol reviews the latest developments in microinjection, with a particular emphasis on quality control and genotyping strategies.
Read Article
Cite this Article
Delerue, F., Ittner, L. M. Generation of Genetically Modified Mice through the Microinjection of Oocytes. J. Vis. Exp. (124), e55765, doi:10.3791/55765 (2017).
Copy