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Generation of Genetically Modified Mice through the Microinjection of Oocytes
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Generation of Genetically Modified Mice through the Microinjection of Oocytes
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10:19 min
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June 15, 2017
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The microinjection of mouse oocytes is commonly used for both classic transgenesis (i.e., the random integration of transgenes) and CRISPR-mediated gene targeting. This protocol reviews the latest developments in microinjection, with a particular emphasis on quality control and genotyping strategies.
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Delerue, F., Ittner, L. M. Generation of Genetically Modified Mice through the Microinjection of Oocytes. J. Vis. Exp. (124), e55765, doi:10.3791/55765 (2017).
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