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Generation of Genetically Modified Mice through the Microinjection of Oocytes
JoVE Journal
Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Generation of Genetically Modified Mice through the Microinjection of Oocytes

Generation of Genetically Modified Mice through the Microinjection of Oocytes

20,082 Views

10:19 min

June 15, 2017

DOI:

10:19 min
June 15, 2017

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Summary

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The microinjection of mouse oocytes is commonly used for both classic transgenesis (i.e., the random integration of transgenes) and CRISPR-mediated gene targeting. This protocol reviews the latest developments in microinjection, with a particular emphasis on quality control and genotyping strategies.

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