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适用于下一代测序的植物细菌DNA富集方法的优化与比较分析
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Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
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适用于下一代测序的植物细菌DNA富集方法的优化与比较分析

DOI:
10.3791/55528-v

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12:33 min

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July 28, 2017

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, ,
1Cereal Disease Laboratory,United States Department of Agriculture-Agricultural Research Service, 2Department of Horticultural Science,University of Minnesota, 3Department of Plant Pathology,University of Minnesota

Chapters

  • 00:05Title
  • 00:49Growing Wheat Plants
  • 01:51Organellar DNA Enrichment by Differential Centrifugation (DC)
  • 07:31Organellar DNA Enrichment by Methyl-fractionation (MF)
  • 09:44Results: Both Enrichment Methods Yield Sufficient DNA Highly Enriched for Organellar Sequences and Suitable for NGS
  • 11:33Conclusion

Summary

자동 번역

자동 번역

提出了两种植物细菌DNA富集方法的比较和优化：传统差速离心和基于甲基化状态的总gDNA分馏。我们评估产生的DNA数量和质量，证明在短读下一代测序中的性能，并讨论长读单分子测序中使用的潜力。

Tags

Plant OrganelleDNA EnrichmentNext-generation SequencingDifferential CentrifugationMeta FractionationWheat SeedlingOrganelle IsolationSTE BufferFiltration

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