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Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
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Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

DOI:
10.3791/55528-v

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12:33 min

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July 28, 2017

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, ,
1Cereal Disease Laboratory,United States Department of Agriculture-Agricultural Research Service, 2Department of Horticultural Science,University of Minnesota, 3Department of Plant Pathology,University of Minnesota

Capítulos

  • 00:05Título
  • 00:49Growing Wheat Plants
  • 01:51Organellar DNA Enrichment by Differential Centrifugation (DC)
  • 07:31Organellar DNA Enrichment by Methyl-fractionation (MF)
  • 09:44Results: Both Enrichment Methods Yield Sufficient DNA Highly Enriched for Organellar Sequences and Suitable for NGS
  • 11:33Conclusion

Summary

Traducción Automática

Traducción Automática

The comparison and optimization of two plant organellar DNA enrichment methods are presented: traditional differential centrifugation and fractionation of the total gDNA based on methylation status. We assess the resulting DNA quantity and quality, demonstrate performance in short-read next-generation sequencing, and discuss the potential for use in long-read single-molecule sequencing.

Tags

Plant OrganelleDNA EnrichmentNext-generation SequencingDifferential CentrifugationMeta FractionationWheat SeedlingOrganelle IsolationSTE BufferFiltration

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