Journal
/
Genética
/
Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
JoVE Journal
Genética
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Genética
Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

12,730 Views

•

12:33 min

•

July 28, 2017

DOI:

10.3791/55528-v

DOI:
10.3791/55528-v

•

12:33 min
July 28, 2017

•

16 Views
, ,
1Cereal Disease Laboratory,United States Department of Agriculture-Agricultural Research Service, 2Department of Horticultural Science,University of Minnesota, 3Department of Plant Pathology,University of Minnesota

Summary

Automatically generated

The comparison and optimization of two plant organellar DNA enrichment methods are presented: traditional differential centrifugation and fractionation of the total gDNA based on methylation status. We assess the resulting DNA quantity and quality, demonstrate performance in short-read next-generation sequencing, and discuss the potential for use in long-read single-molecule sequencing.

Explore More Videos

Plant OrganelleDNA EnrichmentNext-generation SequencingDifferential CentrifugationMeta FractionationWheat SeedlingOrganelle IsolationSTE BufferFiltration

Read Article

Cite this Article

Miller, M. E., Liberatore, K. L., Kianian, S. F. Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing. J. Vis. Exp. (125), e55528, doi:10.3791/55528 (2017).

Download .ris file

Copy

Share Video

.

Copy