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Genetics

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JoVE Journal Genetics
Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
 

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Article DOI: 10.3791/55528-v • 12:33 min • July 28th, 2017
July 28th, 2017 •
*1,2, *1,3, 1,3
1Cereal Disease Laboratory, United States Department of Agriculture-Agricultural Research Service, 2Department of Horticultural Science, University of Minnesota, 3Department of Plant Pathology, University of Minnesota
* These authors contributed equally

Capitoli

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The comparison and optimization of two plant organellar DNA enrichment methods are presented: traditional differential centrifugation and fractionation of the total gDNA based on methylation status. We assess the resulting DNA quantity and quality, demonstrate performance in short-read next-generation sequencing, and discuss the potential for use in long-read single-molecule sequencing.

Tags

Optimization Comparative Analysis Plant Organellar DNA Enrichment Methods Next-generation Sequencing Plastid DNA Mitochondria DNA Plant Biology Organellar Diversity Organellar Genome Sequence Organellar Structure Development Stress Response Differential Centrifugation Method Meta Fractionation Technique Frozen Tissue Samples Wheat Seedlings Vermiculite Square Pots Greenhouse Light Cycle Water Plants Fertilize Plants Granular 20-20-20 MPK Fertilizer Germination Adulation Of Wheat Seedlings Dark Growth Chamber Ventilation
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