Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

Marisa E. Miller; Katie L. Liberatore; Shahryar F. Kianian

doi:10.3791/55528

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Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

JoVE Journal
遺伝学

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JoVE Journal 遺伝学

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

12,723 Views

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12:33 min

•

July 28, 2017

DOI:

10.3791/55528-v

DOI:

10.3791/55528-v

•

12:33 min

July 28, 2017

•

16 Views

Marisa E. Miller*^1,2, Katie L. Liberatore*^1,3, Shahryar F. Kianian^1,3

¹Cereal Disease Laboratory,United States Department of Agriculture-Agricultural Research Service, ²Department of Horticultural Science,University of Minnesota, ³Department of Plant Pathology,University of Minnesota

概要

The comparison and optimization of two plant organellar DNA enrichment methods are presented: traditional differential centrifugation and fractionation of the total gDNA based on methylation status. We assess the resulting DNA quantity and quality, demonstrate performance in short-read next-generation sequencing, and discuss the potential for use in long-read single-molecule sequencing.

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Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing

概要

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