JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing
8,098 Views
•
07:41 min
•
February 04, 2017
DOI:
1Department of Medicine, Unit of Computational Medicine,Karolinska Institutet, 2Center for Molecular Medicine,Karolinska Institutet, 3Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry 2,Karolinska Institutet, 4Department of Medicine,University of California San Diego
Summary
Automatically generated
This article describes a method for studying cellular metabolism in complex communities of multiple cell types, using a combination of stable isotope tracing, cell sorting to isolate specific cell types, and mass spectrometry.
Read Article
Cite this Article
Roci, I., Gallart-Ayala, H., Watrous, J., Jain, M., Wheelock, C. E., Nilsson, R. A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing. J. Vis. Exp. (120), e55011, doi:10.3791/55011 (2017).
Copy