Waiting
Procesando inicio de sesión ...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / A Method for Measuring Metabolism in Sorted…
JoVE Journal
Biochemistry

Se requiere una suscripción a JoVE para ver este contenido. Inicie sesión o comience su prueba gratuita.

JoVE Journal Biochemistry
A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing
 

A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing

Article DOI: 10.3791/55011-v • 07:41 min • February 4th, 2017
February 4th, 2017 •
1,2, 3, 4, 4, 3, 1,2
1Department of Medicine, Unit of Computational Medicine, Karolinska Institutet, 2Center for Molecular Medicine, Karolinska Institutet, 3Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry 2, Karolinska Institutet, 4Department of Medicine, University of California San Diego

Capítulos

Resumen
Automatic Translation

This article describes a method for studying cellular metabolism in complex communities of multiple cell types, using a combination of stable isotope tracing, cell sorting to isolate specific cell types, and mass spectrometry.

Tags

Metabolism Sorted Subpopulations Complex Cell Communities Stable Isotope Tracing Metabolic Differences Metabolic Collaboration Cell Sorting Reliable Measure Of Metabolism Visual Demonstration Metabolite Extraction Culture Cells Isotope Labeled Culture Medium Dialyzed Supplements Cold HBSS Dry Ice Precooled Methanol Mass Spectrometry Analysis
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter