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A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing
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JoVE Journal 生化学
A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing

A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing

DOI:
10.3791/55011-v

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07:41 min

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February 04, 2017

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, , , , ,
1Department of Medicine, Unit of Computational Medicine,Karolinska Institutet, 2Center for Molecular Medicine,Karolinska Institutet, 3Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry 2,Karolinska Institutet, 4医学部,University of California San Diego

章

  • 00:05標題
  • 00:52Metabolite Extraction from a Dish
  • 01:53Metabolite Extraction of Sorted Cell
  • 04:26Data Analysis
  • 05:22Results: Representative Mas Isotopomer Distribution Validation and Cell Cycle Phase Difference
  • 06:50Conclusion

概要

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自動翻訳

This article describes a method for studying cellular metabolism in complex communities of multiple cell types, using a combination of stable isotope tracing, cell sorting to isolate specific cell types, and mass spectrometry.

タグ

Metabolite ExtractionCell SortingStable Isotope Tracing代謝Cell SubpopulationsCell CultureMass Spectrometry

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