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A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing
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生化学
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JoVE Journal 生化学
A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing

A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing

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07:41 min

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February 04, 2017

DOI:

10.3791/55011-v

DOI:
10.3791/55011-v

•

07:41 min
February 04, 2017

•

3 Views
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1Department of Medicine, Unit of Computational Medicine,Karolinska Institutet, 2Center for Molecular Medicine,Karolinska Institutet, 3Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry 2,Karolinska Institutet, 4医学部,University of California San Diego

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This article describes a method for studying cellular metabolism in complex communities of multiple cell types, using a combination of stable isotope tracing, cell sorting to isolate specific cell types, and mass spectrometry.

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Metabolite ExtractionCell SortingStable Isotope Tracing代謝Cell SubpopulationsCell CultureMass Spectrometry

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Cite this Article

Roci, I., Gallart-Ayala, H., Watrous, J., Jain, M., Wheelock, C. E., Nilsson, R. A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing. J. Vis. Exp. (120), e55011, doi:10.3791/55011 (2017).

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