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A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing
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JoVE Journal Biyokimya
A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing

A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing

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07:41 min

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February 04, 2017

DOI:

10.3791/55011-v

DOI:
10.3791/55011-v

•

07:41 min
February 04, 2017

•

3 Views
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1Department of Medicine, Unit of Computational Medicine,Karolinska Institutet, 2Center for Molecular Medicine,Karolinska Institutet, 3Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry 2,Karolinska Institutet, 4Tıp Fakültesi,University of California San Diego

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This article describes a method for studying cellular metabolism in complex communities of multiple cell types, using a combination of stable isotope tracing, cell sorting to isolate specific cell types, and mass spectrometry.

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Metabolite ExtractionCell SortingStable Isotope TracingMetabolizmaCell SubpopulationsCell CultureMass Spectrometry

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Roci, I., Gallart-Ayala, H., Watrous, J., Jain, M., Wheelock, C. E., Nilsson, R. A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing. J. Vis. Exp. (120), e55011, doi:10.3791/55011 (2017).

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