00:53Inoculation of Mice with Clostridium difficile
03:22Enumeration of C. difficile from Mouse Fecal Content
05:03Results: The Murine Model of C. difficile Infection Demonstrates Significant Weight Loss, Bacterial Colonization and Histopathological Changes in Infected Mice
This protocol outlines the cefoperazone mouse model of Clostridium difficile infection (CDI) using a clinically-relevant and genetically-tractable strain, R20291. Emphasis on clinical disease monitoring, C. difficile bacterial enumeration, toxin cytotoxicity, and histopathological changes throughout CDI in a mouse model are detailed in the protocol.