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Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

12,055 Views

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06:51 min

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December 10, 2016

DOI:

10.3791/54850-v

DOI:
10.3791/54850-v

•

06:51 min
December 10, 2016

•

5 Views
, , ,
1Department of Population Health and Pathobiology,North Carolina State University College of Veterinary Medicine, 2Department of Pathology and Laboratory Medicine, Lineberger Comprehensive Cancer Center,University of North Carolina School of Medicine

Summary

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This protocol outlines the cefoperazone mouse model of Clostridium difficile infection (CDI) using a clinically-relevant and genetically-tractable strain, R20291. Emphasis on clinical disease monitoring, C. difficile bacterial enumeration, toxin cytotoxicity, and histopathological changes throughout CDI in a mouse model are detailed in the protocol.

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Cite this Article

Winston, J. A., Thanissery, R., Montgomery, S. A., Theriot, C. M. Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291. J. Vis. Exp. (118), e54850, doi:10.3791/54850 (2016).

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