Journal
/
Imunologia e Infecção
/
Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

12,056 Views

•

06:51 min

•

December 10, 2016

DOI:

10.3791/54850-v

DOI:
10.3791/54850-v

•

06:51 min
December 10, 2016

•

5 Views
, , ,
1Department of Population Health and Pathobiology,North Carolina State University College of Veterinary Medicine, 2Department of Pathology and Laboratory Medicine, Lineberger Comprehensive Cancer Center,University of North Carolina School of Medicine

Summary

Automatically generated

This protocol outlines the cefoperazone mouse model of Clostridium difficile infection (CDI) using a clinically-relevant and genetically-tractable strain, R20291. Emphasis on clinical disease monitoring, C. difficile bacterial enumeration, toxin cytotoxicity, and histopathological changes throughout CDI in a mouse model are detailed in the protocol.

Explore More Videos

Clostridium DifficileMouse ModelCefoperazoneSpore InoculumGavageAnaerobicDilutionEnumerationToxin CytotoxicityHistopathology

Read Article

Cite this Article

Winston, J. A., Thanissery, R., Montgomery, S. A., Theriot, C. M. Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291. J. Vis. Exp. (118), e54850, doi:10.3791/54850 (2016).

Download .ris file

Copy

Share Video

.

Copy