ジャーナル
/
免疫学と感染
/
Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291
JoVE Journal
免疫学と感染
このコンテンツを視聴するには、JoVE 購読が必要です。  サインイン又は無料トライアルを申し込む。
JoVE Journal 免疫学と感染
Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

DOI:
10.3791/54850-v

•

06:51 min

•

December 10, 2016

•

, , ,
1Department of Population Health and Pathobiology,North Carolina State University College of Veterinary Medicine, 2Department of Pathology and Laboratory Medicine, Lineberger Comprehensive Cancer Center,University of North Carolina School of Medicine

章

  • 00:05標題
  • 00:53Inoculation of Mice with Clostridium difficile
  • 03:22Enumeration of C. difficile from Mouse Fecal Content
  • 05:03Results: The Murine Model of C. difficile Infection Demonstrates Significant Weight Loss, Bacterial Colonization and Histopathological Changes in Infected Mice
  • 06:10Conclusion

概要

自動翻訳

自動翻訳

This protocol outlines the cefoperazone mouse model of Clostridium difficile infection (CDI) using a clinically-relevant and genetically-tractable strain, R20291. Emphasis on clinical disease monitoring, C. difficile bacterial enumeration, toxin cytotoxicity, and histopathological changes throughout CDI in a mouse model are detailed in the protocol.

タグ

Clostridium DifficileMouse ModelCefoperazoneSpore InoculumGavageAnaerobicDilutionEnumerationToxin CytotoxicityHistopathology

Article

Embed

プレイリスト追加

Usage Statistics

関連ビデオ

Culturing and Maintaining Clostridium difficile in an Anaerobic Environment

A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment

Influenza A Virus Studies in a Mouse Model of Infection

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection

A Purification and In Vitro Activity Assay for a (p)ppGpp Synthetase from Clostridium difficile

A Controlled Mouse Model for Neonatal Polymicrobial Sepsis

Measurement of Pulse Propagation Velocity, Distensibility and Strain in an Abdominal Aortic Aneurysm Mouse Model

Constructing Mutants in Serotype 1 Streptococcus pneumoniae strain 519/43

Experimental Autoimmune Uveitis: An Intraocular Inflammatory Mouse Model

Mouse Model of Oleic Acid-Induced Acute Respiratory Distress Syndrome

Read Article