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Chapters
- 00:05Title
- 01:12Culturing Microglial Cells
- 03:47Measuring Cytokine Release from Microglial Cells
- 05:42Using Cultured Hypothalamic Cells for a Toxicity Assay
- 06:57Results: Cytokine Release from Microglial after AgNP Exposure
- 07:54Conclusion
Microglia (immune cells of the brain), are used as a surrogate biosensor to determine how nanoparticles influence neurotoxicity. We describe a series of experiments designed to assay microglial response to nanoparticles and exposure of hypothalamic neurons to supernatant from activated microglia to determine neurotoxicity.
