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Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity
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神经科学
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JoVE 杂志 神经科学
Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity

Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity

DOI:
10.3791/54662-v

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08:37 min

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October 25, 2016

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, , , , ,
1Minneapolis Veterans Affairs Health Care System, 2Department of Food Science and Nutrition,University of Minnesota, 3Biomedical Informatics and Computational Biology Program,University of Minnesota, 4Minnesota Obesity Center,University of Minnesota

Chapters

  • 00:05Title
  • 01:12Culturing Microglial Cells
  • 03:47Measuring Cytokine Release from Microglial Cells
  • 05:42Using Cultured Hypothalamic Cells for a Toxicity Assay
  • 06:57Results: Cytokine Release from Microglial after AgNP Exposure
  • 07:54Conclusion

Summary

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自动翻译

Microglia (immune cells of the brain), are used as a surrogate biosensor to determine how nanoparticles influence neurotoxicity. We describe a series of experiments designed to assay microglial response to nanoparticles and exposure of hypothalamic neurons to supernatant from activated microglia to determine neurotoxicity.

Tags

MicrogliaBiosensorNanoparticleNeurotoxicity神经科学In VitroCell CultureBV2 CellsSilver NanoparticlesConditioned MediaFiltrationCell CountingCell SeedingCell StarvationCell Activation

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