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Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity
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JoVE Journal Neurowissenschaften
Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity

Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity

6,832 Views

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08:37 min

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October 25, 2016

DOI:

10.3791/54662-v

DOI:
10.3791/54662-v

•

08:37 min
October 25, 2016

•

1 Views
, , , , ,
1Minneapolis Veterans Affairs Health Care System, 2Department of Food Science and Nutrition,University of Minnesota, 3Biomedical Informatics and Computational Biology Program,University of Minnesota, 4Minnesota Obesity Center,University of Minnesota

Summary

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Microglia (immune cells of the brain), are used as a surrogate biosensor to determine how nanoparticles influence neurotoxicity. We describe a series of experiments designed to assay microglial response to nanoparticles and exposure of hypothalamic neurons to supernatant from activated microglia to determine neurotoxicity.

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Cite this Article

Duffy, C. M., Ahmed, S., Yuan, C., Mavanji, V., Nixon, J. P., Butterick, T. Microglia as a Surrogate Biosensor to Determine Nanoparticle Neurotoxicity. J. Vis. Exp. (116), e54662, doi:10.3791/54662 (2016).

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