Journal
/
면역학 및 감염병학
/
Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes

Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes

8,636 Views

•

08:21 min

•

July 28, 2016

DOI:

10.3791/54293-v

DOI:
10.3791/54293-v

•

08:21 min
July 28, 2016

•

4 Views
, ,
1Department of Microbiology and Immunology,University of Michigan Medical School, 2Department of Biophysics,University of Michigan

Summary

Automatically generated

We illustrate here an in vitro membrane binding assay in which interactions between HIV-1 Gag and lipid membranes are visually analyzed using YFP-tagged Gag synthesized in a wheat germ-based in vitro translation system and GUVs prepared by an electroformation technique.

Explore More Videos

HIV-1 GagGiant Unilamellar Vesicle (GUV)Membrane BindingLipid CompositionVirostructural ProteinsElectroformationITO-coated SlidesLipid Film FormationChloroformTemperature Control

Read Article

Cite this Article

Olety, B., Veatch, S. L., Ono, A. Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes. J. Vis. Exp. (113), e54293, doi:10.3791/54293 (2016).

Download .ris file

Copy

Share Video

.

Copy