Journal
/
Immunologie und Infektion
/
Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes
JoVE Journal
Immunologie und Infektion
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Immunologie und Infektion
Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes

Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes

8,636 Views

•

08:21 min

•

July 28, 2016

DOI:

10.3791/54293-v

DOI:
10.3791/54293-v

•

08:21 min
July 28, 2016

•

4 Views
, ,
1Department of Microbiology and Immunology,University of Michigan Medical School, 2Department of Biophysics,University of Michigan

Summary

Automatically generated

We illustrate here an in vitro membrane binding assay in which interactions between HIV-1 Gag and lipid membranes are visually analyzed using YFP-tagged Gag synthesized in a wheat germ-based in vitro translation system and GUVs prepared by an electroformation technique.

Explore More Videos

HIV-1 GagGiant Unilamellar Vesicle (GUV)Membrane BindingLipid CompositionVirostructural ProteinsElectroformationITO-coated SlidesLipid Film FormationChloroformTemperature Control

Read Article

Cite this Article

Olety, B., Veatch, S. L., Ono, A. Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes. J. Vis. Exp. (113), e54293, doi:10.3791/54293 (2016).

Download .ris file

Copy

Share Video

.

Copy