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Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes

Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes

8,636 Views

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08:21 min

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July 28, 2016

DOI:

10.3791/54293-v

DOI:
10.3791/54293-v

•

08:21 min
July 28, 2016

•

4 Views
, ,
1Department of Microbiology and Immunology,University of Michigan Medical School, 2Department of Biophysics,University of Michigan

Summary

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We illustrate here an in vitro membrane binding assay in which interactions between HIV-1 Gag and lipid membranes are visually analyzed using YFP-tagged Gag synthesized in a wheat germ-based in vitro translation system and GUVs prepared by an electroformation technique.

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Cite this Article

Olety, B., Veatch, S. L., Ono, A. Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes. J. Vis. Exp. (113), e54293, doi:10.3791/54293 (2016).

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