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Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes
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Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes
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July 28, 2016
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We illustrate here an in vitro membrane binding assay in which interactions between HIV-1 Gag and lipid membranes are visually analyzed using YFP-tagged Gag synthesized in a wheat germ-based in vitro translation system and GUVs prepared by an electroformation technique.
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Olety, B., Veatch, S. L., Ono, A. Visualization of HIV-1 Gag Binding to Giant Unilamellar Vesicle (GUV) Membranes. J. Vis. Exp. (113), e54293, doi:10.3791/54293 (2016).
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