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Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow
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Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow
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July 28, 2016
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The protocol describes a novel murine femur window chamber model that can be used to track movement of cells in the femoral bone marrow in vivo. Intravital multiphoton fluorescence microscopy is used to image three components of the femoral bone marrow (vasculature, collagen matrix, and neutrophils) over time.
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Chen, Y., Maeda, A., Bu, J., DaCosta, R. Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow. J. Vis. Exp. (113), e54205, doi:10.3791/54205 (2016).
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