Journal
/
Inmunología y contagio
/
Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow
JoVE Revista
Inmunología y contagio
Se requiere una suscripción a JoVE para ver este contenido.  Inicie sesión o comience su prueba gratuita.
JoVE Revista Inmunología y contagio
Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow

Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow

8,026 Views

•

08:41 min

•

July 28, 2016

DOI:

10.3791/54205-v

DOI:
10.3791/54205-v

•

08:41 min
July 28, 2016

•

1 Views
, , ,
1Princess Margaret Cancer Centre, 2Department of Medical Biophysics,University of Toronto, 3Techna Institute,University Health Network

Summary

Automatically generated

The protocol describes a novel murine femur window chamber model that can be used to track movement of cells in the femoral bone marrow in vivo. Intravital multiphoton fluorescence microscopy is used to image three components of the femoral bone marrow (vasculature, collagen matrix, and neutrophils) over time.

Explore More Videos

Femur Window ChamberIn Vivo Cell TrackingMurine Bone MarrowNeutrophil ImagingVascular NetworkHematopoiesisImmunologyOncologyTumorigenesisTumor MetastasisAthymic Nude MouseSurgical ProcedureMicroscopyMultiphotonConfocal

Read Article

Cite this Article

Chen, Y., Maeda, A., Bu, J., DaCosta, R. Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow. J. Vis. Exp. (113), e54205, doi:10.3791/54205 (2016).

Download .ris file

Copy

Share Video

.

Copy