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Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow
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Immunology and Infection
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JoVE Journal Immunology and Infection
Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow

Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow

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8,026 Views

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08:41 min

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July 28, 2016

DOI:

10.3791/54205-v

DOI:
10.3791/54205-v

•

08:41 min
July 28, 2016

•

1 Views
, , ,
1Princess Margaret Cancer Centre, 2Department of Medical Biophysics,University of Toronto, 3Techna Institute,University Health Network

Summary

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The protocol describes a novel murine femur window chamber model that can be used to track movement of cells in the femoral bone marrow in vivo. Intravital multiphoton fluorescence microscopy is used to image three components of the femoral bone marrow (vasculature, collagen matrix, and neutrophils) over time.

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Chen, Y., Maeda, A., Bu, J., DaCosta, R. Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow. J. Vis. Exp. (113), e54205, doi:10.3791/54205 (2016).

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