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Immunology and Infection

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JoVE Journal Immunology and Infection
Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow
 

Femur Window Chamber Model for In Vivo Cell Tracking in the Murine Bone Marrow

Article DOI: 10.3791/54205-v • 08:41 min • July 28th, 2016
July 28th, 2016 •
*1, *1,2, 1, 1,2,3
1Princess Margaret Cancer Centre, 2Department of Medical Biophysics, University of Toronto, 3Techna Institute, University Health Network
* These authors contributed equally

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The protocol describes a novel murine femur window chamber model that can be used to track movement of cells in the femoral bone marrow in vivo. Intravital multiphoton fluorescence microscopy is used to image three components of the femoral bone marrow (vasculature, collagen matrix, and neutrophils) over time.

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Femur Window Chamber Model In Vivo Cell Tracking Murine Bone Marrow Chronic Window Chamber Neutrophils Vascular Structures Immunology Oncology Monocytes Infection Regulation Tumorigenesis Tumor Metastasis Hematopoiesis Conventional Methods Immune Cell Populations Sedation Level Confirmation Toe Pinch Test Electric Heating Pad Surgical Drape Stereo Microscope Ointment Application Surgical Area Sterilization Skin Incision Lateral Side Approach
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