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Use of Shigella flexneri to Study Autophagy-Cytoskeleton Interactions
Chapters
- 00:05Title
- 01:58Infecting Cells with Shigella flexneri
- 05:06Pharmacological Treatment of Infected HeLa Cells
- 06:05Preparations for Injecting S. flexneri into Zebrafish Larvae
- 07:30Injecting S. flexneri into Zebrafish Larvae and Survival Analysis
- 09:17Injecting S. flexneri into Zebrafish Larvae and Live Imaging
- 11:53Conclusion
To counteract pathogen dissemination, host cells reorganize their cytoskeleton to compartmentalize bacteria and induce autophagy. Using Shigella infection of tissue culture cells, host and pathogen determinants underlying this process are identified and characterized. Using zebrafish models of Shigella infection, the role of discovered molecules and mechanisms are investigated in vivo.
