Journal
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Immunologie et infection
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Use of Shigella flexneri to Study Autophagy-Cytoskeleton Interactions
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Use of Shigella flexneri to Study Autophagy-Cytoskeleton Interactions

Use of Shigella flexneri to Study Autophagy-Cytoskeleton Interactions

DOI:
10.3791/51601-v

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12:52 min

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September 09, 2014

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, ,
1Section of Microbiology, MRC Centre for Molecular Bacteriology and Infection,Imperial College London, 2Département de Biologie du Développement et des Cellules Souches,Institut Pasteur, Unité Macrophages et Développement de l’Immunité

Chapitres

  • 00:05Titre
  • 01:58Infecting Cells with Shigella flexneri
  • 05:06Pharmacological Treatment of Infected HeLa Cells
  • 06:05Preparations for Injecting S. flexneri into Zebrafish Larvae
  • 07:30Injecting S. flexneri into Zebrafish Larvae and Survival Analysis
  • 09:17Injecting S. flexneri into Zebrafish Larvae and Live Imaging
  • 11:53Conclusion

Summary

Traduction automatique

Traduction automatique

To counteract pathogen dissemination, host cells reorganize their cytoskeleton to compartmentalize bacteria and induce autophagy. Using Shigella infection of tissue culture cells, host and pathogen determinants underlying this process are identified and characterized. Using zebrafish models of Shigella infection, the role of discovered molecules and mechanisms are investigated in vivo.

Tags

Shigella FlexneriAutophagyCytoskeletonActinSeptinCell Autonomous ImmunityZebrafish

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