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Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun
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Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun

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12:02 min

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April 18, 2014

DOI:

10.3791/51234-v

DOI:
10.3791/51234-v

•

12:02 min
April 18, 2014

•

17 Views
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1Synthetic Biology Platform, Wyss Institute for Biologically Inspired Engineering,Harvard University, 2Department of Systems Biology,Harvard Medical School, 3Department of Biotechnology,Delft University of Technology

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This work describes a novel method for selectively targeting subcellular organelles in plants, assayed using the BioRad Gene Gun.

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Alternative SplicingChloroplastCytosolGibson AssemblyGFPGene GunMetabolic EngineeringMulti-organelle TargetingPeroxisomeSynthetic BiologyTobaccoTransient Gene Expression

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Mattozzi, M. D., Voges, M. J., Silver, P. A., Way, J. C. Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun. J. Vis. Exp. (86), e51234, doi:10.3791/51234 (2014).

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