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Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun
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Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun

20,216 Views

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12:02 min

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April 18, 2014

DOI:

10.3791/51234-v

DOI:
10.3791/51234-v

•

12:02 min
April 18, 2014

•

17 Views
, , ,
1Synthetic Biology Platform, Wyss Institute for Biologically Inspired Engineering,Harvard University, 2Department of Systems Biology,Harvard Medical School, 3Department of Biotechnology,Delft University of Technology

Summary

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This work describes a novel method for selectively targeting subcellular organelles in plants, assayed using the BioRad Gene Gun.

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Alternative SplicingChloroplastCytosolGibson AssemblyGFPGene GunMetabolic EngineeringMulti-organelle TargetingPeroxisomeSynthetic BiologyTobaccoTransient Gene Expression

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Cite this Article

Mattozzi, M. D., Voges, M. J., Silver, P. A., Way, J. C. Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun. J. Vis. Exp. (86), e51234, doi:10.3791/51234 (2014).

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