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Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun

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Environnement

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Journal JoVE Environnement

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun

20,216 Views

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12:02 min

•

April 18, 2014

DOI:

10.3791/51234-v

DOI:

10.3791/51234-v

•

12:02 min

April 18, 2014

•

17 Views

Matthew D. Mattozzi^1,2, Mathias J. Voges^1,2,3, Pamela A. Silver^1,2, Jeffrey C. Way^1,2

¹Synthetic Biology Platform, Wyss Institute for Biologically Inspired Engineering,Harvard University, ²Department of Systems Biology,Harvard Medical School, ³Department of Biotechnology,Delft University of Technology

Summary

This work describes a novel method for selectively targeting subcellular organelles in plants, assayed using the BioRad Gene Gun.

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