/

/

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun

JoVE Journal
Ambiente

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Ambiente

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun

Transient Gene Expression in Tobacco using Gibson Assembly and the Gene Gun

20,216 Views

•

12:02 min

•

April 18, 2014

DOI:

10.3791/51234-v

DOI:

10.3791/51234-v

•

12:02 min

April 18, 2014

•

17 Views

Matthew D. Mattozzi^1,2, Mathias J. Voges^1,2,3, Pamela A. Silver^1,2, Jeffrey C. Way^1,2

¹Synthetic Biology Platform, Wyss Institute for Biologically Inspired Engineering,Harvard University, ²Department of Systems Biology,Harvard Medical School, ³Department of Biotechnology,Delft University of Technology

Summary

This work describes a novel method for selectively targeting subcellular organelles in plants, assayed using the BioRad Gene Gun.

Explore More Videos

Alternative Splicing Chloroplast Cytosol Gibson Assembly GFP Gene Gun Metabolic Engineering Multi-organelle Targeting Peroxisome Synthetic Biology Tobacco Transient Gene Expression

Read Article