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Procedures for Identifying Infectious Prions After Passage Through the Digestive System of an Avian Species
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:56Crow Gavaging
- 05:21Mouse Inoculation and Monitoring
- 08:05Confirmation of Scrapie Diagnosis
- 10:18Results: Western Blot Analysis Confirms Diagnosis in Mice
- 11:30Conclusion
Scavengers have potential to translocate infectious transmissible spongiform encephalopathy prions in their feces to disease-free areas. We detail methods used to determine if mouse-adapted scrapie prions remain infectious after passage though the digestive tract of American crows (Corvus brachyrhynchos), a common consumer of dead animals.
