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Procedures for Identifying Infectious Prions After Passage Through the Digestive System of an Avian Species
Chapters
- 00:05Title
- 01:56Crow Gavaging
- 05:21Mouse Inoculation and Monitoring
- 08:05Confirmation of Scrapie Diagnosis
- 10:18Results: Western Blot Analysis Confirms Diagnosis in Mice
- 11:30Conclusion
Scavengers have potential to translocate infectious transmissible spongiform encephalopathy prions in their feces to disease-free areas. We detail methods used to determine if mouse-adapted scrapie prions remain infectious after passage though the digestive tract of American crows (Corvus brachyrhynchos), a common consumer of dead animals.
