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Procedures for Identifying Infectious Prions After Passage Through the Digestive System of an Avian Species
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Procedures for Identifying Infectious Prions After Passage Through the Digestive System of an Avian Species

Procedures for Identifying Infectious Prions After Passage Through the Digestive System of an Avian Species

DOI:

12:00 min

November 06, 2013

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Capítulos

  • 00:05Título
  • 01:56Crow Gavaging
  • 05:21Mouse Inoculation and Monitoring
  • 08:05Confirmation of Scrapie Diagnosis
  • 10:18Results: Western Blot Analysis Confirms Diagnosis in Mice
  • 11:30Conclusion

Summary

Tadução automática

Scavengers have potential to translocate infectious transmissible spongiform encephalopathy prions in their feces to disease-free areas. We detail methods used to determine if mouse-adapted scrapie prions remain infectious after passage though the digestive tract of American crows (Corvus brachyrhynchos), a common consumer of dead animals.

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