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Surface Passivation for Single-molecule Protein Studies
JoVE 신문
화학
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JoVE 신문 화학
Surface Passivation for Single-molecule Protein Studies

Surface Passivation for Single-molecule Protein Studies

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10:35 min

April 24, 2014

DOI:

10:35 min
April 24, 2014

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Summary

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We describe a method for passivating a glass surface using polyethylene glycol (PEG). This protocol covers surface cleaning, surface functionalization, and PEG coating. We introduce a new strategy for treating the surface with PEG molecules over two rounds, which yields superior quality of passivation compared to existing methods.

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