We describe a method for passivating a glass surface using polyethylene glycol (PEG). This protocol covers surface cleaning, surface functionalization, and PEG coating. We introduce a new strategy for treating the surface with PEG molecules over two rounds, which yields superior quality of passivation compared to existing methods.
Chandradoss, S. D., Haagsma, A. C., Lee, Y. K., Hwang, J., Nam, J., Joo, C. Surface Passivation for Single-molecule Protein Studies. J. Vis. Exp. (86), e50549, doi:10.3791/50549 (2014).