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Surface Passivation for Single-molecule Protein Studies
JoVE Journal
Kimya
This content is Free Access.
JoVE Journal Kimya
Surface Passivation for Single-molecule Protein Studies

Surface Passivation for Single-molecule Protein Studies

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10:35 min

April 24, 2014

DOI:

10:35 min
April 24, 2014

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We describe a method for passivating a glass surface using polyethylene glycol (PEG). This protocol covers surface cleaning, surface functionalization, and PEG coating. We introduce a new strategy for treating the surface with PEG molecules over two rounds, which yields superior quality of passivation compared to existing methods.

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