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Surface Passivation for Single-molecule Protein Studies
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Chimie
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Journal JoVE Chimie
Surface Passivation for Single-molecule Protein Studies

Surface Passivation for Single-molecule Protein Studies

DOI:
10.3791/50549-v

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10:35 min

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April 24, 2014

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, , , , ,
1Kavli Institute of NanoScience, Department of BioNanoScience,Delft University of Technology, 2Department of Chemistry,Seoul National University

Chapitres

  • 00:05Titre
  • 01:35Slide Preparation and Cleaning
  • 04:08Amino-silanization of Slides and Coverslips
  • 05:24Surface Passivation Using Polymer (The First Round)
  • 07:30Assembling a Microfluidic Chamber
  • 08:52Results: Quality Control
  • 10:06Conclusion

Summary

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We describe a method for passivating a glass surface using polyethylene glycol (PEG). This protocol covers surface cleaning, surface functionalization, and PEG coating. We introduce a new strategy for treating the surface with PEG molecules over two rounds, which yields superior quality of passivation compared to existing methods.

Tags

Single-moleculeProteinSurface PassivationFluorescence SpectroscopyPEG CoatingSurface CleaningSurface FunctionalizationPiranha EtchingSurface Immobilization

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