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Surface Passivation for Single-molecule Protein Studies
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Chemistry
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JoVE Journal Chemistry
Surface Passivation for Single-molecule Protein Studies

Surface Passivation for Single-molecule Protein Studies

English

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40,769 Views

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10:35 min

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April 24, 2014

DOI:

10.3791/50549-v

DOI:
10.3791/50549-v

•

10:35 min
April 24, 2014

•

32 Views
, , , , ,
1Kavli Institute of NanoScience, Department of BioNanoScience,Delft University of Technology, 2Department of Chemistry,Seoul National University

Summary

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We describe a method for passivating a glass surface using polyethylene glycol (PEG). This protocol covers surface cleaning, surface functionalization, and PEG coating. We introduce a new strategy for treating the surface with PEG molecules over two rounds, which yields superior quality of passivation compared to existing methods.

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Cite this Article

Chandradoss, S. D., Haagsma, A. C., Lee, Y. K., Hwang, J., Nam, J., Joo, C. Surface Passivation for Single-molecule Protein Studies. J. Vis. Exp. (86), e50549, doi:10.3791/50549 (2014).

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