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면역학 및 감염병학

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使用骨髓源性巨噬细胞巨噬细胞极化的调查

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

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使用骨髓源性巨噬细胞巨噬细胞极化的调查

DOI:

10.3791/50323-v

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10:07 min

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June 23, 2013

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Wei Ying, Patali S. Cheruku, Fuller W. Bazer, Stephen H. Safe, Beiyan Zhou

¹Department of Animal Science,Texas A&M University, ²Department of Biology,Texas A&M University, ³Department of Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences,Texas A&M University

Chapters

00:05Title
01:36Isolation of Bone Marrow Cells
03:23Induction BMDM Formation
04:17BMDM Polarized Activation
06:11Representative Analysis of BMDM
09:19Conclusion

Summary

자동 번역

本文介绍了一种自适应容易容易<em>在体外</em>模型，探讨巨噬细胞极化。在存在下GM-CSF/M-CSF，从骨髓中的造血干/祖细胞定向分化成单核细胞，由M1或M2的刺激。激活状态可以跟踪的细胞表面抗原，基因表达和细胞信号转导通路的变化。

Tags

Macrophage Polarization Bone Marrow Derived Macrophages GM-CSF M-CSF Monocytic Differentiation M1 Polarization M2 Polarization Cell Surface Antigens Gene Expression Cell Signaling Pathways

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