Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

Wei Ying; Patali S. Cheruku; Fuller W. Bazer; Stephen H. Safe; Beiyan Zhou

doi:10.3791/50323

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免疫学と感染

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Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

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免疫学と感染

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JoVE Journal 免疫学と感染

Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

64,809 Views

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10:07 min

•

June 23, 2013

DOI:

10.3791/50323-v

DOI:

10.3791/50323-v

•

10:07 min

June 23, 2013

•

68 Views

Wei Ying¹, Patali S. Cheruku², Fuller W. Bazer¹, Stephen H. Safe³, Beiyan Zhou³

¹Department of Animal Science,Texas A&M University, ²生物学科,Texas A&M University, ³Department of Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences,Texas A&M University

概要

The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways.

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Macrophage Polarization Bone Marrow Derived Macrophages GM-CSF M-CSF Monocytic Differentiation M1 Polarization M2 Polarization Cell Surface Antigens Gene Expression Cell Signaling Pathways

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Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages

概要

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