/
/
Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages
Bu içeriği görüntülemek için JoVE aboneliği gereklidir. Oturum açın veya ücretsiz deneme sürümünü başlatın.
Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages
64,995 Views
•
10:07 min
•
June 23, 2013
DOI:
Özet
Automatically generated
The article describes a readily easy adaptive in vitro model to investigate macrophage polarization. In the presence of GM-CSF/M-CSF, hematopoietic stem/progenitor cells from the bone marrow are directed into monocytic differentiation, followed by M1 or M2 stimulation. The activation status can be tracked by changes in cell surface antigens, gene expression and cell signaling pathways.
Read Article
Cite this Article
Ying, W., Cheruku, P. S., Bazer, F. W., Safe, S. H., Zhou, B. Investigation of Macrophage Polarization Using Bone Marrow Derived Macrophages. J. Vis. Exp. (76), e50323, doi:10.3791/50323 (2013).
Copy