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过敏性气道炎症在肺树突状细胞的成熟和迁移分析
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JoVE 신문 면역학 및 감염병학
Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation
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过敏性气道炎症在肺树突状细胞的成熟和迁移分析

DOI:
10.3791/4014-v

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07:52 min

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July 23, 2012

•

,
1Stem Cell and Cancer Research Institute,McMaster University, Hamilton, 2Physiology and Experimental Medicine Research Program, Hospital for Sick Children,University of Toronto

Chapters

  • 00:05Title
  • 01:24Sensitizing and Intranasal Challenge of Mice with Ovalbumin
  • 02:39Preparation of Single-cell Suspension from the Lungs and the Draining Lymph Nodes
  • 05:37Results: OVA Sensitization and Challenge Induces an Increase in CD11c+ Cell Trafficking to Murine Mediastinal Lymph Nodes
  • 07:33Conclusion

Summary

자동 번역

자동 번역

我们描述了一个战略，以监测肺部树突状细胞的成熟和迁移的卵在卵蛋白引起的过敏性气道炎症的设置。这种策略可以进行修改，以评估肺部树突状细胞的迁移，在感染的设置。

Tags

Pulmonary Dendritic CellsMaturationMigrationAllergic Airway InflammationAdaptive Immune ResponseAntigen-specific T CellsAntigen StimulationDraining Lymph NodesT Cell ResponseAutoimmune ResponseAllergic Immune ResponseImmune ToleranceLung EnvironmentEnvironmental AntigensAllergyAsthmaAirway DC MaturationAirway DC MigrationSensitizationOvalbumin-induced Allergic Asthma

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