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Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation
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Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation
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July 23, 2012
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We describe a strategy to monitor maturation and migration of pulmonary dendritic cells in response to ovalbumin in the setting of ovalbumin induced allergic airway inflammation. This strategy can be modified to assess migration of pulmonary dendritic cells in settings of infection.
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Pulmonary Dendritic CellsMaturationMigrationAllergic Airway InflammationAdaptive Immune ResponseAntigen-specific T CellsAntigen StimulationDraining Lymph NodesT Cell ResponseAutoimmune ResponseAllergic Immune ResponseImmune ToleranceLung EnvironmentEnvironmental AntigensAllergyAsthmaAirway DC MaturationAirway DC MigrationSensitizationOvalbumin-induced Allergic Asthma
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Kushwah, R., Hu, J. Analysis of Pulmonary Dendritic Cell Maturation and Migration during Allergic Airway Inflammation. J. Vis. Exp. (65), e4014, doi:10.3791/4014 (2012).
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